rea621  (Miltenyi Biotec)

Journal: Frontiers in Immunology

Article Title: Polyfunctional T cells and unique cytokine clusters imprint the anti rAAV2/rAAV9 vector immune response

doi: 10.3389/fimmu.2024.1450524

Figure Lengend Snippet: Used antibodies with respective conjugations (conj.) and dilutions for the flow-cytometric analyses.

Article Snippet: CD134 , PE-Vio770 , 1:50 , REA621 , 130-120-723 , Miltenyi.

Techniques:

Journal: bioRxiv

Article Title: Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells

doi: 10.1101/2022.06.17.496630

Figure Lengend Snippet: A-D. Distribution of absolute value differences between technical replicate values for cytokine (TNF, IL-10, and IFN), and high-throughput flow cytometry results (CD80/86, HLA-DR. OX-40), respectively. Each dot represents an individual absolute value difference between technical replicates. Red lines indicate the quantile lines for the absolute value differences for each donor. E. Distribution of the coefficient of variation (CV) for positive control compounds (R848 and CpG ODN) across all study participants. Dotted lines represent thresholds of CV quality measures. Dashed lines demarkate typically accepted thresholds for excellent (<10), good (<30), acceptable (<50), and poor (>50) CV values.

Article Snippet: After the wash, an antibody cocktail consisting of Miltenyi REAfinity Recombinant antibodies HLA-DR Ox40 APC REA621 (Bergisch Gladbach, Germany) was diluted 1:100 in PBS and 5 μL were added to each well using the Combi reagent dispenser.

Techniques: High Throughput Screening Assay, Flow Cytometry, Positive Control

Journal: bioRxiv

Article Title: Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells

doi: 10.1101/2022.06.17.496630

Figure Lengend Snippet: A. Distribution of z scores, indicating induction of the cytokines (IFN, IL-10, and TNF). B. Distribution of z scores, representing the percentage of monocytes with activated HLA-DR and/or CD80/86 receptors. C. Distribution of z scores, representing the percentage of T-Cells with activated Ox40 receptors. D. Distribution of z scores, representing the percentage of B-Cells with activated HLA-DR and/or CD80/86 receptors. E. graphical representation of small molecule hit rate in varying number of donors. natural log of hit rate. F. Strong negative correlation between number of donors. Each dot represents one of the 2,296 compounds from the primary screen. The dashed line represents the donor threshold of a z score of 2 and representative z scores were chosen such that any compound above the threshold represents a hit in the screen.

Article Snippet: After the wash, an antibody cocktail consisting of Miltenyi REAfinity Recombinant antibodies HLA-DR Ox40 APC REA621 (Bergisch Gladbach, Germany) was diluted 1:100 in PBS and 5 μL were added to each well using the Combi reagent dispenser.

Techniques:

Journal: bioRxiv

Article Title: Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells

doi: 10.1101/2022.06.17.496630

Figure Lengend Snippet: A. Distribution of z scores, representing the percentage of monocytes with activated HLA-DR and/or CD80/86 receptors. The dashed line represents the hit threshold of a z score of 2. All receptor combinations must be increased for a compound to be classified as a confirmed hit. B. Distribution of z scores, representing production of IFN, IL-10, and TNF. The dashed line represents the hit threshold of a z score of 2. C. Rank order of mean cytokine induction (± SEM) at 11 μM for the 19 compounds selected for the multiplexed concentration titration assay based on the results of the monocyte receptor activation and cytokine induction in the confirmation experiment.

Article Snippet: After the wash, an antibody cocktail consisting of Miltenyi REAfinity Recombinant antibodies HLA-DR Ox40 APC REA621 (Bergisch Gladbach, Germany) was diluted 1:100 in PBS and 5 μL were added to each well using the Combi reagent dispenser.

Techniques: Concentration Assay, Titration, Activation Assay

Journal: bioRxiv

Article Title: Adjuvant Discovery via a High Throughput Screen using Human Primary Mononuclear Cells

doi: 10.1101/2022.06.17.496630

Figure Lengend Snippet: Key resource table

Article Snippet: After the wash, an antibody cocktail consisting of Miltenyi REAfinity Recombinant antibodies HLA-DR Ox40 APC REA621 (Bergisch Gladbach, Germany) was diluted 1:100 in PBS and 5 μL were added to each well using the Combi reagent dispenser.

Techniques: Recombinant, Sterility, Injection, Modification, Staining, Saline, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Software